Repozytorium Uniwersytetu
Andrzeja Frycza Modrzewskiego
w Krakowie
Witaj w Repozytorium Uniwersytetu
Andrzeja Frycza Modrzewskiego w Krakowie
Repozytorium eRIKA to cyfrowa kolekcja dokumentów stanowiących świadectwo naukowej, badawczej oraz dydaktycznej działalności pracowników Uniwersytetu Andrzeja Frycza Modrzewskiego w Krakowie. Znajdziesz tu między innymi: artykuły naukowe, książki i fragmenty książek, rozprawy doktorskie, raporty z prac badawczych, nagradzane prace magisterskie i licencjackie oraz materiały konferencyjne. A wszystko w otwartym dostępie! Zbiory w DSpace
Wybierz Zbiór, aby przeglądać jego Kolekcje
Teraz wyświetlane 1 - 5 z 5
Najnowsze publikacje
FOS and Other Genes Linked to Oxidative Stress Are Upregulated in Women Suffering from Endometriosis and Might Negatively Impact the Oocyte Developmental Competence During ICSI
(MDPI, 2026-07-17) Kordowitzki, Pawel; Kochan, Joanna; Wyroba, Jakub
Abstract: „Purpose: We aim to elucidate the impact of endometriomas on oocyte developmental competence and identify genetic alterations that may influence ICSI success rates in women of different ages. Methods: In this retrospective study, two groups of patients have been analyzed: 108 women with a history of severe endometriosis (stages III and IV), who underwent OPU and ICSI after endometriosis surgery, and a control group, built out of 108 women with no endometriosis history. The Turku Endometriosis Database was used for differential gene expression analysis. Results: The number of aspirated oocytes (endometrioma group: 2.75 ± 0.32 vs. control 10.32 ± 0.54) was significantly (p < 0.0001) lower in patients with endometrioma excision, and the number of generated blastocysts was significantly (p < 0.0001) lower when compared to patients without endometriosis (endometrioma group: 1.09 ± 0.13 vs. control group: 3.76 ± 0.23). Additionally, AMH levels were significantly lower (p < 0.001) in the endometriosis-affected group below the age of 35 years (1.65 ± 0.15) than in the unaffected control group (2.99 ± 0.27) within the same age group. The mRNA expression of the following genes related to oxidative stress was significantly (p < 0.05) upregulated in the endometriosis group compared with healthy controls: DES, P2RX1, FOS, SRF, RASD2, SLITRK3, and TEAD3. Gene Ontology and pathway enrichment analyses demonstrated a significant (p < 0.0001) enrichment of oxidative stress–associated biological processes and signaling pathways, including responses to reactive oxygen species and hypoxia, HIF-1, MAPK, PI3K–Akt, NF-κB, and apoptotic signaling, supporting oxidative stress as a central molecular mechanism underlying the observed transcriptomic alterations. Conclusions: In conclusion, this retrospective study provides a holistic perspective on the impact of endometrioma and its oxidative stress effect on oocyte developmental rates and ICSI outcomes across different age groups.”(…)
“Old and new” frontiers in embryo viability assessment: current practices and introduction of emerging approaches
(Oxford University Press, 2026-07-14) Singh, Sareena Rayne; Abass, Madeline; Bartlewski, Jakub; Kochan, Joanna; Murawski, Maciej; Wyroba, Jakub; Bartlewski, Pawel M.
Abstract: „Reliable assessment of embryo viability remains a critical yet challenging component of assisted reproductive technologies (ART). Traditional methods, including morphological grading and preimplantation genetic testing for aneuploidy (PGT-A), are either subjective, invasive, or resource intensive. Consequently, non-invasive approaches, including analysis of spent culture medium (SCM) and digital image-based analytical methods, emerged as promising alternatives to support clinical decision-making, though their reliability varies. SCM analyses can detect embryonic DNA and metabolites, but their outcomes are constrained by potential contamination with non-embryonic material and inconsistent amplification efficiency. In contrast, image-based assessment combined with machine learning (ML) enables quantitative, repeatable, and transparent evaluation of embryo architecture. Segmental image analysis of grayscale bitmaps allows for extraction of interpretable phototextural metrics, which have been correlated with developmental competence and ploidy status of embryos. Recent applications of ML-assisted techniques demonstrate comparable or superior predictive performance relative to PGT-A and expert embryologists, highlighting their potential as cost-effective and ethically favorable alternatives. Continued refinement of image acquisition minimizing artifacts, optimizing quality and resolution, and focusing on key regions such as the trophectoderm and inner cell mass, may further enhance their diagnostic sensitivity and specificity. Overall, computerized assessment of embryo microphotographs represents a scalable, non-invasive strategy that can complement or even partially replace invasive testing. By leveraging structured, interpretable image data, algorithms such as r-Algo 2.0 (discriminative image-analysis software) exemplify the emerging role of computational tools in advancing diagnostic precision and objectivity in ART.
Highlights:
Current methods of embryo viability assessment lack clinical reliability
Non-invasive image analysis supports accurate embryo quality assessment
Transparent algorithms enhance repeatability of image-analysis techniques
Image-based embryo assessments may complement or reduce the need for PGT-A
Imaging refinements can further enhance its diagnostic sensitivity and specificity
Summary Sentence:
Computerized image analysis and machine learning offer a non-invasive, scalable, and reliable alternative to currently used embryo assessment methods, with comparable or improved predictive performance, enhancing objectivity and clinical decisionmaking in ART.”(…)
Success Rates of Assisted Reproduction in Couples with Poor Ovarian Response and Oligospermia
(MDPI, 2025-09-24) Wyroba, Jakub; Kochan, Joanna; Brązert, Maciej; Kordowitzki, Paweł
Abstract: „Recent progress in assisted reproductive medicine has introduced novel therapeutic possibilities for couples experiencing various reproductive challenges or subfertility. A critical concern in this field is the diminished ovarian response to hormonal treatments preceding ovum pickup, necessitating personalised and optimised protocols to enhance ovarian response across different age groups. Furthermore, a common male factor in IVF couples, oligozoospermia, characterised by a low sperm count, significantly impacts the success rates of assisted reproductive technologies, posing an increasing challenge for in vitro fertilisation clinics. Lifestyle choices, dietary habits, and overall health behaviours have also demonstrably affected fertility outcomes in the 21st century. This original article aims to highlight the synergistic importance of both partners’ health, specifically addressing poor ovarian response and oligozoospermia, in achieving successful conception. Our study analysed intracytoplasmic sperm injection outcomes in couples affected by both aforementioned conditions and proposed an optimal management strategy. This study shows that oligozoospermia significantly reduced ICSI fertilisation and cleavage rates. Poor ovarian responders experienced more cancelled cycles due to fewer embryos. While blastocyst rates relative to zygotes were comparable, overall success was lower in groups with male factor infertility and poor ovarian response, necessitating personalised treatment approaches.”(…)
Anti-Müllerian Hormone Concentrations in Women of Different Reproductive Age and the Chances of IVF Outcome: A Paradigm Shift is needed
(ISOAD, 2026-02-01) Wyroba, Jakub; Kochan, Joanna; Kelley, Liam; Iqbal, Sharif; Kordowitzki, Pawel
Abstract: „The study of Anti-Müllerian Hormone (AMH) has garnered considerable attention due to its critical implications in assessing and understanding both female and male fertility potential. Traditionally, AMH is recognized for its pivotal role in evaluating ovarian reserve and is a cornerstone in reproductive health assessments for women. The aim of this study was to challenge the traditional interpretation of AMH as a standalone predictor of IVF success. Through a retrospective analysis of 600 patients undergoing ICSI, we reveal that women with low AMH levels, traditionally classified as poor responders, can achieve unexpectedly high oocyte numbers, blastocyst formation, and pregnancy rates. This highlights the limitations of using AMH alone to predict IVF outcomes. Our findings advocate the importance of integrating additional factors, such as follicle-stimulating hormone (FSH), and the need for a more individualized approach to fertility treatment planning.”(…)
Diminished ovarian reserve (DOR) — a step-by-step overview of the IVF procedure based on current knowledge
(Via Medica Journals, 2026-07-30) Wyroba, Jakub; Imiolek, Magdalena; Krol-Mika, Andzelika; Kiecka, Kamil; Wojtowicz, Teresa; Kostarczyk, Katarzyna; Kochan, Joanna
Abstract: „Review of current knowledge of the in vitro fertilization (IVF) procedure in patients with diminished ovarian reserve (DOR). Patients with DOR who undergo IVF require special care and an individualized approach due to the poor prognosis. The IVF procedure is multi-stage, and each step should be carefully planned, with treatments tailored to DOR patients.
The review article was prepared based on an analysis of the latest research findings and ESHRE recommendations for all stages of in vitro fertilization in patients with DOR. The article discusses the results of 32 articles published over the last 5 years, including 23 articles published in 2024–2026. Clinical stages such as prediction of ovarian reserve, hormonal stimulation of the ovaries, and embryo transfer, and laboratory stages such as the quality and quantity of obtained oocytes and embryos, results of preimplantation genetic testing (PGT-A), and finally implantation potential and live birth rate in DOR patients were discussed.”(…)