Przeglądaj wg Autor "Grzywacz, Agata"

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    Anti-inflammatory properties of cantharellus cibarius from in vitro culture enriched in zinc
    (Polish Pharmaceutical Society, 2018) Gudula-Argasińska, Joanna; Grzywacz, Agata; Krakowska, Agata; Opoka, Włodzimierz; Muszyńska, Bożena
    Cantharellus cibarius Fr. (Basidiomycota), commonly known as Chanterelle, is one of the most valued and currently most often collected species of edible mushrooms in Europe, Asia, Africa, and the northern USA. Cantharellus genus (chanterelles) includes seventy species, seven of which are noted in Europe. Due to the valuable and biologically active metabolite content in the fruiting bodies, C. cibarius was selected for the study. The combinations of carbohydrates, amino acids, unsaturated fatty acids, and vitamins revealed the nutritional, in particular, anti-inflammatory properties of this species. Zinc (Zn) is one of the microelements present in significant quantities in the fruiting bodies and biomass from in vitro cultures of edible mushrooms. Zn plays a crucial role as an anti-inflammatory agent. This paper presents a study on investigation of the pro- or antiinflammatory properties of mushroom extracts from fruiting bodies and biomass from in vitro culture of C. cibarius enriched in Zn. As an additive to the modified Oddoux liquid medium, on which in vitro C. cibarius cultures were grown, the following two compounds were used: zinc sulfate and zinc hydroxyaspartate. In order to compare which complex will most effectively increase the anti-inflammatory activity of C. cibarius, inorganic and organic compounds were used. Both zinc salts were used in such quantities that the calculated additive amount of zinc into the culture media was the same. Control cultures were represented by in vitro cultures on Oddoux medium grown without the supplementation of zinc compounds. In addition, we studied which source of zinc is best for enhancing anti-inflammatory properties in A549 (Human Lung Epithelial Carcinoma, ATCC) cell models activated with lipopolysaccharide (LPS) to induce inflammation. Results of the study showed the important role of zinc during inflammation in the lung epithelial cells. Cantharellus cibarius in vitro cultures with high ability to accumulate zinc enabled the precise application of zinc compounds at a known concentration that may influence their immuno-modulatory properties.
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    Composition and Biological Properties of Agaricus bisporus Fruiting Bodies – a Review
    (http://journal.pan.olsztyn.pl, 2017) Muszyńska, Bożena; Kała, Katarzyna; Rojowski, Jacek; Grzywacz, Agata; Opoka, Włodzimierz
    White Agaricus bisporus is both the most popular and the most commonly eaten edible mushroom species in the world. It is popular not only because of its taste, but also due to its high level of nutrients: dietary fiber (chitin), essential, semi-essential amino acids, unsaturated fatty acids including linoleic and linolenic acids, easily digestible proteins, sterols, phenolic and indole compounds, and vitamins − especially provitamin D2 and B1, B2, B6, B7, and C. Fruiting bodies of A. bisporus have antioxidant, antibacterial, anti-inflammatory, antitumor, and immunomodulatory activity. The presence of antioxidant ergothioneine (which also displays the antimutagenic, chemo- and radioprotective activity) is also noteworthy. A. bisporus also contains derivatives of benzoquinone, a substance which belongs to the group of antibiotics. Studies of tyrosinase isolated from this species show its very high similarity to human tyrosinase. This points directly to the fact that this species could be a rich source of tyrosinase used for medicinal and cosmetics purposes. A. bisporus is also a rich source of selenium, zinc and other elements such as magnesium, copper, iron, potassium, sodium, calcium, phosphorus, sulfur or manganese. In conclusion, the presence of these compounds and elements with biological activity in fruiting bodies of A. bisporus confirms their nutraceutical and medicinal properties.
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    Resolvin D2 plays a protective role in RAW 264.7 Cells treated with polycyclic aromatic hydrocarbons
    (Polish Academy of Sciences – Cracow Branch, 2015) Gdula-Argasińska, Joanna; Woźniakiewicz, Aneta; Woźniakiewicz, Michał; Lipkowska, Anna; Olbert, Magdalena; Grzywacz, Agata; Sałat, Kinga; Podkowa, Adrian; Librowski, Tadeusz
    Eicosanoids are signaling molecules that control the immune processes and might have effects on inflammatory diseases. The aim of our study was to evaluate the effect of added resolvin D2 (RvD2), after treatment with polycyclic aromatic hydrocarbons (PAHs), on RAW 264.7 cells by using a UHPLC/MS-TOF method for the quantification of eicosanoids: 8-iPGF3α, PGF3α, 8-isoPGF2α, PGF2α and 5-iPF2α as well as cyclooxygenase 2 (COX-2), prostaglandin E synthase (cPGES) and prostaglandin F2α (FP) receptor protein expression by Western blot. The levels of PGF3α, PGF2α 8-iPGF3α 8-isoPGF2α and 5-iPF2α were decreased in RAW 264.7 cells after the exposure to PAHs and treatment with RvD2. It was observed that COX-2, cPGES and FP-receptor expression was decreased after co-treatment of the cells with PAHs and RvD2. Our findings suggest that RvD2 has anti-oxidant, anti-inflammatory and pro-resolving properties that may contribute significantly to alleviation of the harmful effects caused by PAHs in macrophages. Moreover, these results suggest that a diet rich in n-3 fatty acids might be helpful in resolving the inflammation and mitigating the effects of environmental stress in macrophages.
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    The role of zinc in the pathogenesis and treatment of central nervous system (CNS) diseases. implications of zinc homeostasis for proper cns function
    (Polish Pharmaceutical Society, 2014) Tyszka-Czochara, Małgorzata; Grzywacz, Agata; Gdula-Argasińska, Joanna; Librowski, Tadeusz; Wiliński, Bogdan; Opoka, Włodzimierz
    Zinc, the essential trace element, is known to play multiple biological functions in human organism. This metal is a component of many structural as well as regulatory and catalytic proteins. The precise regulation of zinc homeostasis is essential for central nervous system and for the whole organism. Zinc plays a significant role in the brain development and in the proper brain function at every stage of life. This article is a review of knowledge about the role of zinc in central nervous system (CNS) function. The influence of this biometal on etiopathogenesis, prevention and treatment of selected brain diseases and disorders was discussed. Zinc imbalance can result not only from insufficient dietary intake, but also from impaired activity of zinc transport proteins and zinc dependent regulation of metabolic pathways. It is known that some neurodegenerative processes are connected with zinc dyshomeostasis and it may influence the state of Alzheimerís disease, depression and ageing-connected loss of cognitive function. The exact role of zinc and zinc-binding proteins in CNS pathogenesis processes is being under intensive investigation. The appropriate zinc supplementation in brain diseases may help in the prevention as well as in the proper treatment of several brain dysfunctions.