Przeglądaj wg Autor "Rak, Agnieszka"

Teraz wyświetlane 1 - 2 z 2
  • Miniatura
    Pozycja
    Adropin in polycystic ovarian syndrome: expression and impact on human granulosa cells function
    (BioScientifica Limited, 2026-08-14) Kurowska, Patrycja; Dawid, Monika; Respekta-Długosz, Natalia; Oprocha, Julia; Szkraba, Oliwia; Skrzypski, Marek; Couty, Noémie; Ramé, Christelle; Guérif, Fabrice; Wyroba, Jakub; Kochan, Joanna; Gajda, Lechosław; Trzcińska, Monika; Dupont, Joelle; Rak, Agnieszka
    Abstract: „Adropin is a novel protein that regulates energy homeostasis. Serum and follicular fluid (FF) levels of adropin are decreased in women with polycystic ovarian syndrome (PCOS); however, its role in ovarian function remains unknown. The aims of this study were to determine the expression of adropin and its receptor G protein-coupled receptor 19 (GPR19), in human granulosa cells (GC), its immunolocalization, and its in vitro effects on GC function. Blood plasma, FF, and GC samples were obtained from normal-weight, obese, and women diagnosed with or without PCOS (n = 8). The in vitro effects of adropin on GC proliferation, apoptosis, cell cycle progression, and steroidogenesis were analyzed. The results revealed that adropin plasma concentration was decreased in obese patients, with a similar reduction observed in obese patients with PCOS, whereas GPR19 expression was decreased in the GC of obese and PCOS women, as well as in obese patients with PCOS. We noted that in all investigated patient groups, adropin reduced GC proliferation and cell cycle progression, negatively influenced steroidogenic enzyme levels, and promoted apoptosis. Such disruptions in GC function are likely to impair ovarian follicular maturation and contribute to the subfertility commonly observed in PCOS. These alterations may ultimately affect oocyte competence and ovarian responsiveness, parameters that are clinically relevant for in vitro fertilization outcomes. Our findings suggest that adropin may act as a novel regulator of ovarian function and could contribute to the pathophysiology of PCOS, highlighting its potential clinical value as a marker of altered ovarian follicular function in affected women.”(…)
  • Miniatura
    Pozycja
    New aspect on the regulation of in vitro oocyte maturation: role of the obesity, neuropeptides and adipokines
    (Springer Nature, 2024-12-13) Kurowska, Patrycja; Wyroba, Jakub; Pich, Karolina; Respekta-Długosz, Natalia; Szkraba, Oliwia; Greggio, Aleksandra; Kochan, Joanna; Rak, Agnieszka
    Abstract: „Oocyte quality determinants and nuclear and cytoplasmic maturation establish essential processes for fertilization and further development of the conceptus. Moreover, female fertility is strongly dependent on the metabolic status of the organism. Numerous sources indicate that obesity impairs ovarian function including oocyte physiology by inhibiting nuclear maturation, stimulating lipotoxicity and inflammation, enabling cumulus cells apoptosis, promoting reactive oxygen species formation and ultimately imposing pathogenic effects on mitochondria leading to infertility. Whereas, the number of overweight and obese individuals has reached alarming levels over the past decades, what is more, by 2030, the prevalence of overweight and obesity might reach 65.3% in adults in China and 78% in the USA. Thus, relationships between reproduction and metabolism are being intensively studied to prevent obesity-induced infertility. The metabolic markers of oocyte condition and function are adipokines and neuropeptides, which regulate food intake, lipid and glucose metabolism, insulin resistance and impart significant influences on reproduction. Thus, in this review, we focus on interrelationships between obesity, oocyte maturation and the role of selected neuropeptides and adipokines including leptin, adiponectin, kisspeptin, nesfatin-1, phoenixin, visfatin, chemerin and vaspin.”(…)