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Pozycja Analysis of the day 3 transfer strategy for POSEIDON patients(SBRA - Associação Brasileira de Reprodução Assistida, 2025-07-30) Wyroba, Jakub; Kochan, JoannaAbstract: „Objective: The aim of the study was to analyze the effectiveness of the day 3 ET strategy, and the morphology of the transferred embryos, in patients from POSEIDON and non-POSEIDON groups. Methods: 600 cycles of patients meeting the POSEIDON criteria and 600 non-POSEIDON cycles were analyzed to determine the proportion of cycles with an ET on days 3 or 5, or FET. Then we reviewed 330 day 3 ETs to compared the developmental stage, morphology, zona pellucida thickness and implantation potential of embryos transferred on day 3 from POSEIDON and non-POSEIDON patients. Results: Most cycles of POSEIDON patients end with ET on day 3 (42%) or without transfer (37%). In contrast, most cycle of non-POSEIDON patients end with FET (44%) and just 9% is canceled. The lowest percentage of embryos at the morula stage was recorded in POSEIDON groups III (10%) and IV (9%). The average number of cells in embryos was comparable in all groups. The largest percentage of top-quality embryos (grade A) were in POSEIDON group I (47%) .The highest implantation potential were observed in the non-POSEIDON group <35Y (28%), and in POSEIDON groups I (28%) and III (26%). The highest incidence of miscarriage was recorded in all POSEIDON and non-POSEIDON groups that included patients who were ≥35 years of age. Conclusions: The day 3 ET strategy still seems optimal for POSEIDON patients. The prognosis depends on which Poseidon group the patient is in. The best prognosis is for group I and the worst for group IV.”(…)Pozycja Adropin in polycystic ovarian syndrome: expression and impact on human granulosa cells function(BioScientifica Limited, 2026-08-14) Kurowska, Patrycja; Dawid, Monika; Respekta-Długosz, Natalia; Oprocha, Julia; Szkraba, Oliwia; Skrzypski, Marek; Couty, Noémie; Ramé, Christelle; Guérif, Fabrice; Wyroba, Jakub; Kochan, Joanna; Gajda, Lechosław; Trzcińska, Monika; Dupont, Joelle; Rak, AgnieszkaAbstract: „Adropin is a novel protein that regulates energy homeostasis. Serum and follicular fluid (FF) levels of adropin are decreased in women with polycystic ovarian syndrome (PCOS); however, its role in ovarian function remains unknown. The aims of this study were to determine the expression of adropin and its receptor G protein-coupled receptor 19 (GPR19), in human granulosa cells (GC), its immunolocalization, and its in vitro effects on GC function. Blood plasma, FF, and GC samples were obtained from normal-weight, obese, and women diagnosed with or without PCOS (n = 8). The in vitro effects of adropin on GC proliferation, apoptosis, cell cycle progression, and steroidogenesis were analyzed. The results revealed that adropin plasma concentration was decreased in obese patients, with a similar reduction observed in obese patients with PCOS, whereas GPR19 expression was decreased in the GC of obese and PCOS women, as well as in obese patients with PCOS. We noted that in all investigated patient groups, adropin reduced GC proliferation and cell cycle progression, negatively influenced steroidogenic enzyme levels, and promoted apoptosis. Such disruptions in GC function are likely to impair ovarian follicular maturation and contribute to the subfertility commonly observed in PCOS. These alterations may ultimately affect oocyte competence and ovarian responsiveness, parameters that are clinically relevant for in vitro fertilization outcomes. Our findings suggest that adropin may act as a novel regulator of ovarian function and could contribute to the pathophysiology of PCOS, highlighting its potential clinical value as a marker of altered ovarian follicular function in affected women.”(…)Pozycja New aspect on the regulation of in vitro oocyte maturation: role of the obesity, neuropeptides and adipokines(Springer Nature, 2024-12-13) Kurowska, Patrycja; Wyroba, Jakub; Pich, Karolina; Respekta-Długosz, Natalia; Szkraba, Oliwia; Greggio, Aleksandra; Kochan, Joanna; Rak, AgnieszkaAbstract: „Oocyte quality determinants and nuclear and cytoplasmic maturation establish essential processes for fertilization and further development of the conceptus. Moreover, female fertility is strongly dependent on the metabolic status of the organism. Numerous sources indicate that obesity impairs ovarian function including oocyte physiology by inhibiting nuclear maturation, stimulating lipotoxicity and inflammation, enabling cumulus cells apoptosis, promoting reactive oxygen species formation and ultimately imposing pathogenic effects on mitochondria leading to infertility. Whereas, the number of overweight and obese individuals has reached alarming levels over the past decades, what is more, by 2030, the prevalence of overweight and obesity might reach 65.3% in adults in China and 78% in the USA. Thus, relationships between reproduction and metabolism are being intensively studied to prevent obesity-induced infertility. The metabolic markers of oocyte condition and function are adipokines and neuropeptides, which regulate food intake, lipid and glucose metabolism, insulin resistance and impart significant influences on reproduction. Thus, in this review, we focus on interrelationships between obesity, oocyte maturation and the role of selected neuropeptides and adipokines including leptin, adiponectin, kisspeptin, nesfatin-1, phoenixin, visfatin, chemerin and vaspin.”(…)Pozycja PGT-A in POSEIDON Patients - perspectives and limitations(SBRA - Associação Brasileira de Reprodução Assistida, 2025-12-10) Wyroba, Jakub; Barszcz, Maria; Fajt, Iwona; Kochan, JoannaAbstract: „Objective: The aim of this study was to determine the likelihood of being able to perform PGT-A, and its results, in each POSEIDON group compared to their age-matched non-POSEIDON group. Methods: This was a retrospective study of 4 groups of POSEIDON patients (n=824) who underwent intracytoplasmic sperm injection (ICSI) The controls were non-POSEIDON patients in two age groups (<35 and ≥35 years old; n=360). Results: The non-POSEIDON <35Y group had the highest number of embryos at the blastocyst stage that could be used for PGT-A (5.1±2/cycle), and the POSEIDON IV group had the fewest (0.6±0.3/cycle). Significantly fewer blastocysts were PGT-A tested in the groups with no indications for PGT-A (i.e. POSEIDON I (26%) and III (28%) and non-POSEIDON <35Y (39%)), compared to the groups with indications (i.e. POSEIDON II (69%), and IV (67%) and non POSEIDON ≥35Y (72%)). The euploidy rate was similar between groups without PGT-A indications (59%-64%) and between groups with indications (35-41%), but was significantly lower in groups with indications (p<0.001). Conclusions: POSEIDON patients are as willing to undergo PGT-A testing as non-POSEIDON patients, despite the poor prognosis. However, the final PGT-A result is very low compared to that in non-POSEIDON patients of the same age. Failure is usually caused by the inability to perform the blastocyst biopsy due to a lack of oocytes or blastocyst-stage embryos, and to a low rate of euploidy in groups ≥35Y.”(…)Pozycja FOS and Other Genes Linked to Oxidative Stress Are Upregulated in Women Suffering from Endometriosis and Might Negatively Impact the Oocyte Developmental Competence During ICSI(MDPI, 2026-07-17) Kordowitzki, Pawel; Kochan, Joanna; Wyroba, JakubAbstract: „Purpose: We aim to elucidate the impact of endometriomas on oocyte developmental competence and identify genetic alterations that may influence ICSI success rates in women of different ages. Methods: In this retrospective study, two groups of patients have been analyzed: 108 women with a history of severe endometriosis (stages III and IV), who underwent OPU and ICSI after endometriosis surgery, and a control group, built out of 108 women with no endometriosis history. The Turku Endometriosis Database was used for differential gene expression analysis. Results: The number of aspirated oocytes (endometrioma group: 2.75 ± 0.32 vs. control 10.32 ± 0.54) was significantly (p < 0.0001) lower in patients with endometrioma excision, and the number of generated blastocysts was significantly (p < 0.0001) lower when compared to patients without endometriosis (endometrioma group: 1.09 ± 0.13 vs. control group: 3.76 ± 0.23). Additionally, AMH levels were significantly lower (p < 0.001) in the endometriosis-affected group below the age of 35 years (1.65 ± 0.15) than in the unaffected control group (2.99 ± 0.27) within the same age group. The mRNA expression of the following genes related to oxidative stress was significantly (p < 0.05) upregulated in the endometriosis group compared with healthy controls: DES, P2RX1, FOS, SRF, RASD2, SLITRK3, and TEAD3. Gene Ontology and pathway enrichment analyses demonstrated a significant (p < 0.0001) enrichment of oxidative stress–associated biological processes and signaling pathways, including responses to reactive oxygen species and hypoxia, HIF-1, MAPK, PI3K–Akt, NF-κB, and apoptotic signaling, supporting oxidative stress as a central molecular mechanism underlying the observed transcriptomic alterations. Conclusions: In conclusion, this retrospective study provides a holistic perspective on the impact of endometrioma and its oxidative stress effect on oocyte developmental rates and ICSI outcomes across different age groups.”(…)Pozycja “Old and new” frontiers in embryo viability assessment: current practices and introduction of emerging approaches(Oxford University Press, 2026-07-14) Singh, Sareena Rayne; Abass, Madeline; Bartlewski, Jakub; Kochan, Joanna; Murawski, Maciej; Wyroba, Jakub; Bartlewski, Pawel M.Abstract: „Reliable assessment of embryo viability remains a critical yet challenging component of assisted reproductive technologies (ART). Traditional methods, including morphological grading and preimplantation genetic testing for aneuploidy (PGT-A), are either subjective, invasive, or resource intensive. Consequently, non-invasive approaches, including analysis of spent culture medium (SCM) and digital image-based analytical methods, emerged as promising alternatives to support clinical decision-making, though their reliability varies. SCM analyses can detect embryonic DNA and metabolites, but their outcomes are constrained by potential contamination with non-embryonic material and inconsistent amplification efficiency. In contrast, image-based assessment combined with machine learning (ML) enables quantitative, repeatable, and transparent evaluation of embryo architecture. Segmental image analysis of grayscale bitmaps allows for extraction of interpretable phototextural metrics, which have been correlated with developmental competence and ploidy status of embryos. Recent applications of ML-assisted techniques demonstrate comparable or superior predictive performance relative to PGT-A and expert embryologists, highlighting their potential as cost-effective and ethically favorable alternatives. Continued refinement of image acquisition minimizing artifacts, optimizing quality and resolution, and focusing on key regions such as the trophectoderm and inner cell mass, may further enhance their diagnostic sensitivity and specificity. Overall, computerized assessment of embryo microphotographs represents a scalable, non-invasive strategy that can complement or even partially replace invasive testing. By leveraging structured, interpretable image data, algorithms such as r-Algo 2.0 (discriminative image-analysis software) exemplify the emerging role of computational tools in advancing diagnostic precision and objectivity in ART. Highlights: Current methods of embryo viability assessment lack clinical reliability Non-invasive image analysis supports accurate embryo quality assessment Transparent algorithms enhance repeatability of image-analysis techniques Image-based embryo assessments may complement or reduce the need for PGT-A Imaging refinements can further enhance its diagnostic sensitivity and specificity Summary Sentence: Computerized image analysis and machine learning offer a non-invasive, scalable, and reliable alternative to currently used embryo assessment methods, with comparable or improved predictive performance, enhancing objectivity and clinical decisionmaking in ART.”(…)Pozycja Success Rates of Assisted Reproduction in Couples with Poor Ovarian Response and Oligospermia(MDPI, 2025-09-24) Wyroba, Jakub; Kochan, Joanna; Brązert, Maciej; Kordowitzki, PawełAbstract: „Recent progress in assisted reproductive medicine has introduced novel therapeutic possibilities for couples experiencing various reproductive challenges or subfertility. A critical concern in this field is the diminished ovarian response to hormonal treatments preceding ovum pickup, necessitating personalised and optimised protocols to enhance ovarian response across different age groups. Furthermore, a common male factor in IVF couples, oligozoospermia, characterised by a low sperm count, significantly impacts the success rates of assisted reproductive technologies, posing an increasing challenge for in vitro fertilisation clinics. Lifestyle choices, dietary habits, and overall health behaviours have also demonstrably affected fertility outcomes in the 21st century. This original article aims to highlight the synergistic importance of both partners’ health, specifically addressing poor ovarian response and oligozoospermia, in achieving successful conception. Our study analysed intracytoplasmic sperm injection outcomes in couples affected by both aforementioned conditions and proposed an optimal management strategy. This study shows that oligozoospermia significantly reduced ICSI fertilisation and cleavage rates. Poor ovarian responders experienced more cancelled cycles due to fewer embryos. While blastocyst rates relative to zygotes were comparable, overall success was lower in groups with male factor infertility and poor ovarian response, necessitating personalised treatment approaches.”(…)Pozycja Anti-Müllerian Hormone Concentrations in Women of Different Reproductive Age and the Chances of IVF Outcome: A Paradigm Shift is needed(ISOAD, 2026-02-01) Wyroba, Jakub; Kochan, Joanna; Kelley, Liam; Iqbal, Sharif; Kordowitzki, PawelAbstract: „The study of Anti-Müllerian Hormone (AMH) has garnered considerable attention due to its critical implications in assessing and understanding both female and male fertility potential. Traditionally, AMH is recognized for its pivotal role in evaluating ovarian reserve and is a cornerstone in reproductive health assessments for women. The aim of this study was to challenge the traditional interpretation of AMH as a standalone predictor of IVF success. Through a retrospective analysis of 600 patients undergoing ICSI, we reveal that women with low AMH levels, traditionally classified as poor responders, can achieve unexpectedly high oocyte numbers, blastocyst formation, and pregnancy rates. This highlights the limitations of using AMH alone to predict IVF outcomes. Our findings advocate the importance of integrating additional factors, such as follicle-stimulating hormone (FSH), and the need for a more individualized approach to fertility treatment planning.”(…)Pozycja Diminished ovarian reserve (DOR) — a step-by-step overview of the IVF procedure based on current knowledge(Via Medica Journals, 2026-07-30) Wyroba, Jakub; Imiolek, Magdalena; Krol-Mika, Andzelika; Kiecka, Kamil; Wojtowicz, Teresa; Kostarczyk, Katarzyna; Kochan, JoannaAbstract: „Review of current knowledge of the in vitro fertilization (IVF) procedure in patients with diminished ovarian reserve (DOR). Patients with DOR who undergo IVF require special care and an individualized approach due to the poor prognosis. The IVF procedure is multi-stage, and each step should be carefully planned, with treatments tailored to DOR patients. The review article was prepared based on an analysis of the latest research findings and ESHRE recommendations for all stages of in vitro fertilization in patients with DOR. The article discusses the results of 32 articles published over the last 5 years, including 23 articles published in 2024–2026. Clinical stages such as prediction of ovarian reserve, hormonal stimulation of the ovaries, and embryo transfer, and laboratory stages such as the quality and quantity of obtained oocytes and embryos, results of preimplantation genetic testing (PGT-A), and finally implantation potential and live birth rate in DOR patients were discussed.”(…)Pozycja Beyond Spherical: Unveiling the Significance of Oval Blastocyst Morphology on Euploidy and Implantation Success(MDPI, 2025-09-19) Wyroba, Jakub; Kuczyńska, Agnieszka; Kasperkowicz, Klaudia; Kostarczyk, Katarzyna; Kordowitzki, Pawel; Kochan, JoannaAbstract: „The selection of the most suitable embryo, based on the morphology and shape, for embryo transfer is a critical aspect of the in vitro fertilization (IVF) process, as its precision can significantly enhance the overall effectiveness of IVF and contribute to a healthy birth. This study aimed to compare the chromosomal status and implantation potential of oval-shaped blastocysts versus normal-shaped blastocysts on day 5 post-ICSI (intracytoplasmic sperm injection). Initially, the frequency of oval blastocysts was assessed by analyzing 1328 embryos from 610 ICSI cycles. Subsequently, 80 patients undergoing ICSI and PGT-A (preimplantation genetic testing for aneuploidy), who had both normal and oval blastocysts in the same cycle, were selected to evaluate the euploid rate relative to blastocyst morphology. Finally, the implantation outcomes of fresh embryo transfers involving oval and normal-shaped blastocysts, neither of which had undergone PGT-A, were analyzed. Half of the blastocysts from each group were transferred after assisted hatching (AH), and the other half were transferred without AH. Blastocyst shape does not appear to correlate with an increased risk of aneuploidy but does influence hatching ability. Following AH, the implantation potential of elongated blastocysts is equivalent to that of normally shaped blastocysts, suggesting AH is beneficial for oval embryos. Consequently, the transfer of oval blastocysts is considered as safe and effective as the transfer of normally shaped embryos.”(…)Pozycja Reproductive aging, preimplantation genetic testing for aneuploidy, and the diameter of blastocysts: does size matter?(Impact Journals (operating as Rapamycin Press LLC), 2025-03-05) Wyroba, Jakub; Kochan, Joanna; Barszcz, Maria; Mirocki, Grzegorz; Kordowitzki, PawelAbstract: „There is no doubt that maternal aging, also known as reproductive aging, can contribute to the increased rates of aneuploidy observed in blastocysts generated from women of advanced age who undergo in vitro fertilization (IVF). Additionally, the hatching process of the blastocyst, which is crucial for successful implantation, may be impaired in aneuploid embryos. Aneuploid embryos often exhibit abnormal cell division and chromosomal distribution, which can lead to disruptions in the hatching process. Due to ethical restrictions, preimplantation genetic testing for aneuploidy (PGT-A) is unavailable in all countries. Therefore, our retrospective study of 502 couples who underwent intracytoplasmic sperm injection (ICSI) aimed to elucidate if embryonic features, such as the ability to hatch and embryonic diameter, could be a reliable estimator for the success rate after embryo transfer, especially for women aged 26–45 years, and for IVF clinics which do not have access to PGT-A. The small hatching blastocysts (Bl. 5) group had a significant (p < 0.001) higher percentage of euploid embryos (≤35 Y- 73%, >35Y- 51%) compared to large (Bl. 4) counterparts (≤35 Y-58%, >35 Y- 38%). In patients aged 34-38 years, we detected 10% more euploid blastocysts in the hatching group than the expanding ones, which was a significant difference (p < 0.05). In conclusion, when selecting non-PGT-A tested embryos for embryo transfer (ET) or frozen embryo transfer (FET), a small hatching blastocyst seems to be a better choice than a large expanded one, especially for advanced-age patients for whom the risk of aneuploidy is higher.”(…)Pozycja Novel multi-omic biomarkers to combat oocyte and ovarian aging(Springer Nature, 2026-02-03) Kordowitzki, Pawel; Joshi, Shakchhi; Gong, Xutong; Ying, Albert Kejun; Wyroba, Jakub; Kochan, Joanna; Haigis, Marcia C.Abstract: „Understanding oocyte and ovarian aging has become critically important, as trends in family planning evolve, with many women choosing to have children later in life. The ovary, a crucial organ in female reproduction, is particularly susceptible to age-related changes and is one of the organs that exhibit functional deterioration most distinctly with age. The aging of female reproductive systems also affects longevity and various health outcomes. A better understanding of both oocyte and ovarian aging will lay the cornerstone to elucidate the phenomenon of longevity in women. Here, clinical data from 400 women of various ages undergoing intracytoplasmic sperm injection (ICSI) have been analyzed, including Anti-Müllerian Hormone (AMH) and Follicle-Stimulating Hormone (FSH) levels, the number of recovered oocytes, blastocyst rates, pregnancy rates, and live birth rates. Our analyses revealed significant differences in the aforementioned rates between patients of young and advanced age. For the biomarker analysis, we further utilised a novel predictive performance of age-associated gene expression signatures for oocyte aging, demonstrating its potential to provide molecular-level insights into oocyte quality over time. By analyzing RNA sequencing data generated from human oocytes of different ages, a genome-wide landscape of age-associated gene expression has been described. Additionally, metabolome profiling has been performed on young and reproductively aged mice, serving as a model for human ovaries. Changes in metabolites of the murine ovaries during aging have been recorded. In conjunction with traditional biomarkers, multiomics data represent a transformative approach in reproductive health, and they may offer personalised risk assessments and interventions to mitigate age-related fertility decline in women. Our metabolome profiling provides a valuable resource for elucidating the metabolomic basis of ovarian aging. Our findings offer novel insights into systemic shifts associated with oocyte and ovarian aging. This integrated approach may unlock new avenues for fertility preservation, ovarian rejuvenation, and assisted reproduction.”(…)Pozycja Perceived Menstrual Irregularities and Premenstrual Syndrome in Relation to Insomnia: Evidence from a Cohort of Student Nurses(MDPI, 2025-10-22) Dimlievych, Anastasiia; Dębska, Grażyna; Grzesik-Gąsior, Joanna; Merklinger-Gruchala, AnnaAbstract: "Background/Objectives: Sleep disorders, particularly insomnia, are increasingly recognized as key determinants of mental health. Disturbances in sleep architecture may exacerbate hormonal dysregulation, contributing to menstrual cycle irregularities and premenstrual syndrome (PMS). The study investigate the relationship between insomnia symptoms, menstrual problems, and PMS among nursing students. Methods: The cross-sectional study was conducted using a web-based survey (CAWI) among 72 female graduate nursing students. The questionnaire included questions about menstrual history, the presence of menstrual disorders, PMS symptoms, and lifestyle and body mass index (BMI). Insomnia was assessed using the Polish version of the Athens Insomnia Scale (AIS), taking ≥8 as the cutoff point. Logistic regression analysis with confounding variables was performed. Results: 70% of participants had PMS symptoms, 19.5% had irregular menstrual cycles, and 86.5% reported problems with menstrual bleeding. The mean AIS score was 10.1 (SD = 4.05). Women with insomnia were almost 4 times more likely to experience PMS symptoms (OR = 3.93; 95% CI 1.14–13.59), more than 7 times more likely to experience bleeding problems (OR = 7.56; 95% CI: 1.51–37.97), and each additional AIS score increased the risk of cycle irregularity by 24% (OR = 1.24, 95% CI 1.01–1.50). Conclusions: The findings indicate a significant association between insomnia symptoms, menstrual disturbances, and PMS, underscoring the complex links between sleep, reproductive, and mental health. Preventive interventions, particularly sleep hygiene education, may serve as an effective strategy to support women’s overall health and well-being."(...)Pozycja Nursing Students’ Perceptions of Health Literacy: A Cross‑Country Comparative Study(Springer Nature, 2025-03-04) Magerčiaková, Mariana; Lochmannová, Alena; Mainz, Denis; Dębska, GrażynaAbstract: „This study examines nursing students' perceptions of health literacy and explores the impact of educational and cultural differences across Slovakia, the Czech Republic, and Poland. Health literacy is a critical component of effective healthcare, particularly for nurses, who serve as key sources of health information for patients. Understanding nursing students' views on health literacy can provide valuable insights for improving educational curricula. A cross-sectional survey was conducted among 140 second-year nursing students from the three countries using a custom-designed questionnaire. The data were analysed using the Kruskal–Wallis test and Dunn’s post hoc test to identify differences in perceptions and understanding. The findings revealed that Polish students demonstrated the highest familiarity with health literacy, followed by Czech and Slovak students. Notable differences emerged in the perceived public health literacy across the three countries. Czech students rated public comprehension the highest, which may reflect the effectiveness of national health literacy initiatives. In contrast, Slovak and Polish students highlighted several areas needing improvement, particularly in managing chronić conditions and understanding treatment side effects. These results underscore the importance of integrating tailored health literacy education into nursing curricula to address regional disparities. Strengthening nursing students’ competencies in this area can contribute to improved patient outcomes and overall public health.”(…)Pozycja Factors Influencing the Information Support Provided by Health Care Professionals to Patients in a Dialysis Center Regarding Kidney Transplantation: A Nationwide Study(Frontiers, 2025-05-14) Kurleto, Paulina; Tomaszek, Lucyna; Milaniak, Irena; Dębska, Grażyna; Turkanik, Edyta; Siekierska, Barbara; Danielewicz, Roman; Dębska-Ślizień, AlicjaAbstract: „For patients undergoing renal replacement therapy, kidney transplantation (KTx) is the preferred therapeutic method. The aim of this study was to investigate selected factors affecting the information support provided by healthcare professional to patients in dialysis center regarding KTx. A multiple logistic regression was carried out to assess the relationship between information support, socio-demographic factors, life satisfaction (Satisfaction with Life Scale), self-esteem (Self-Esteem Scale), perceived self-efficacy (General Self-Efficacy), attitude, knowledge about organ transplantation. Of the 1,093 respondents aged 22–72 years, 501 respondents (45.8%) always informed patients about the possibility of treatment with KTx. Physicians vs. nurses (OR = 1.79; Cl 95%: 1.48–2.16), and those who supported legalization of unspecified living kidney donation in Poland (OR = 1.30; Cl 95%: 1.07–1.59) and believed that blood donation is safe (OR = 1.29; Cl 95%: 1.12–1.47) were more likely to provide informational support. Knowledge level (OR = 1.32; Cl 95%: 1.18–1.47) and self-esteem (OR = 1.06; Cl95%: 1.03–1.10) correlated positively with information support. Male participants were less likely to provide informational support than females (OR = 0.78; Cl 95%: 0.62–0.99). The results reveal inadequate information provided by healthcare professional to patients about KTx. This highlights the urgent need for comprehensive educational programs.”(…)Pozycja Factors associated with the willingness to become a kidney transplant recipient ─ the results from a national cross sectional study(Springer Nature, 2025-10-16) Kurleto, Paulina; Tomaszek, Lucyna; Milaniak, Irena; Dębska, Grażyna; Turkanik, Edyta; Siekierska, Barbara; Danielewicz, Roman; Dębska-Ślizień, Alicja; Pietrzyk, Jacek A.Abstract: "Background Kidney transplantation (KT) is considered the best method of renal replacement therapy for patients with end-stage renal disease, providing them with better treatment outcomes and improved quality of life. Objective The aim of this study was to investigate the impact of sociodemographic/clinical factors upon knowledge and attitude towards KT, and information support on the willingness to undergo KT among Polish dialysis patients. Methods The cross-sectional study was conducted from August 2023 to December 2024 in 99 dialysis centers in Poland in accordance with ethical approval and international research guidelines. The study included a group of 1,523 adult dialysis patients surveyed with the use of a 5-part self-report questionnaire and standardized tools. Results In the studied group 834 subjects (55%) were willing to undergo KT, while 689 (45%) were reluctant. The dialysis patients with greater knowledge about KT, those who discussed with medical staff the possibility of organ donation from a living donor and those who considered receiving a kidney from either a living related or non related donor (if legally permissible), were more likely to express willingness to become kidney transplant recipients. Conversely, those who were older, with longer dialysis treatment timespan and insufficient informational support regarding KT showed negative association with the willingness to consider renal transplantation. Conclusion Findings suggest that Polish dialysis patients have insufficient knowledge about KT. Higher knowledge and awareness were linked to greater willingness to undergo KT."(...)Pozycja Health-promoting behaviour of university students, based on the Health Behaviour Inventory and on selected laboratory indicators of their health status(University of Kalisz, 2025) Dębska, Grażyna; Kowacka, Ewa; Foryś, Zofia; Gołkowski, Filip; Bałdys-Waligórska, AgataAbstract: "Introduction: To limit the deterioration of health later in life, it is important for young adults to adopt principles of healthy lifestyle and of positive health behaviour as early as possible. Aim of the research was to assess health behaviour in correct eating habits (CEH), health preventive measures (HPM), positive mental attitude (PMA), positive health-maintaining practices (PHMP) and in selected laboratory tests. Material and methods: The study concerned 115 young adults, first-year university students. The Health Behaviour Inventory (HBI) questionnaire was used to examine their health behaviour. Laboratory tests included measurements of total cholesterol, low-density lipoprotein, high-density lipoprotein and triglyceride concentrations, and glucose and insulin levels in blood samples. Results: In the study group, the mean HBI General Index value was 4.6 ± 1.8 stens. The highest mean score (3.27 ± 0.81 points) was observed in the PMA subscale (compared with 3.52 ± 0.66 points in the normalized HBI test by Juczyński), while the lowest (3.11 ± 76 points) was found in the CEH subscale (compared with 3.22 ± 0.76 in the HBI test). Statistically significant correlations were noted between insulin level and HBI General Index value (rs = –0.21, p = 0.030) and between insulin level and CEH (rs = –0.29, p = 0.002). Moreover, a statistically significant correlation between level of triglycerides and HPM (rs = –0.20, p = 0.043) was found. Conclusions: Actions should be taken to improve knowledge of positive health behaviour among students. As they enter their new social environment, such as a university, young adults should be offered the opportunity of changing their lifestyle, the acquired health behaviour patterns serving them in the future."(...)Pozycja Predictors of patient safety culture assessment in a public hospital in Poland(Jan Kochanowski University, 2026-03-25) Borodziuk, Gabriela; Dębska, Grazyna; Merklinger-Gruchała, AnnaAbstract: "Introduction: Patient safety is crucial for healthcare quality and patient trust. It involves activities, attitudes, and procedures to minimise medical errors and provide high standards of care. Aim of the research: The aim of the study was to assess patient safety culture from the perspective of medical staff in a hospital, taking into account professional group affiliation and length of service. Material and methods: The research group included N = 78 medical staff members (80% nurses and midwives, 20% doctors). The Polish adaptation of the Hospital Survey on Patient Safety Culture (HSOPSC) was used. Results: Overall patient safety, measured as the average percentage of positive responses across 12 dimensions, was 36.8%. The best-assessed area was “Teamwork within units”, while the lowest was “Hospital management support for patient safety”. Differences were found between nurses/midwives and doctors: physicians rated “Staffing” more positively and reported more adverse events (c2 = 11.16, df = 2, p = 0.004). Employees with higher length of service (> 10 vs. ≤ 10 years) rated “Organizational learning – continuous improvement” higher (p = 0.03). However, length of service was not statistically associated with adverse event reporting frequency (p = 0.71). Employees who believed their mistakes would be used against them assessed overall patient safety more negatively (p < 0.001). Conclusions: The study’s findings can help identify areas for improvement in patient safety culture, enabling targeted solutions to reduce adverse events and improve error reporting processes."(...)Pozycja Chemical safety of Acheta domesticus powders: a cross-country study of trace elements, mycotoxins and feed-derived pesticide residues(De Gruyter, 2026-07-07) Kurek, Jakub Michał; Kowalczewski, Przemysław Łukasz; Stuper-Szablewska, Kinga; Buśko, Maciej; Perczak, Adam; Ruszkowska, Millena; Proch, Jędrzej; Nowicki, Marcin; Niedzielski, Przemysław; Krejpcio, ZbigniewAbstract: "Among edible insect species, the house cricket (Acheta domesticus) has gained particular prominence in commercial insect farming and various food applications. Since insects intended for use as food or feed can bioaccumulate biological and chemical contaminants, rigorous safety monitoring is essential. The objective of this study was to assess the levels of trace elements, mycotoxins, and pesticide residues in commercially available house cricket powders (CP) sourced from six countries: England (CP1), Scotland (CP2), Vietnam (CP3), Thailand (CP4), Canada (CP5), and Poland (CP6). Trace elements (As, Cd, Cr, I, Ni, Se, Zn, Hg, Pb, and Sb) were quantified following microwave digestion of samples in nitric acid, using inductively coupled plasma mass spectrometry (ICP-MS). Mycotoxins, including aflatoxins B1 and B2, fumonisins B1 and B2, ochratoxin A, and trichothecenes such as DON, 3-AcDON, and NIV, were determined using chromatographic methods appropriate for each analyte group, including GC-MS and HPLC with fluorescence detection. Pesticide residues were analysed via liquid chromatography–tandem mass spectrometry (LC-MS/MS). The results indicated that the majority of CP samples exhibited low and relatively safe levels of the targeted con- taminants. However, elevated concentrations were observed in one sample, specifically arsenic (0.35 ± 0.01 mg/ kg d.m.) and boscalid residue (3.95 ± 2.1 mg/kg d.m.). These findings warrant particular attention to the rearing conditions, including feed quality and environmental factors, to minimize potential bioaccumulation risks in future production." (...)Pozycja Wspieranie dzieci w wieku przedszkolnym z grupy ryzyka dysleksji w rozwoju psychomotorycznym jako czynnik wczesnej profilaktyki trudności w nauce czytania i pisania(Uniwersytet Ignatianum w Krakowie Wydział Pedagogiczny, 2026) Kurowska, BarbaraAbstrakt: "Celem artykułu jest ukazanie znaczenia rozwoju psychomotoryczne go oraz oddziaływań pedagogicznych i logopedycznych w kontekście wczesnej profilaktyki trudności w nauce czytania i pisania u dzieci w wieku przedszkolnym z grupy ryzyka dysleksji. Przedmiotem analizy uczyniono zależności między poziomem rozwoju funkcji psychomotorycznych i językowych a gotowością dziecka do podjęcia nauki szkolnej, a także znaczenie wczesnej diagnozy i obserwacji pedagogicznych. Podjęta problematyka wyraża się w pytaniach: jakie symptomy ryzyka dysleksji mogą być identyfikowane na etapie edukacji przed- szkolnej oraz w jaki sposób działania nauczycieli i specjalistów mogą wspierać rozwój dziecka i ograniczać trudności edukacyjne? Rozważania koncentrują się wokół trzech obszarów. Pierwszy obejmuje charakterystykę rozwoju psychomotorycznego jako fundamentu nabywania umiejętności szkolnych. Drugi dotyczy symptomów ryzyka dysleksji oraz znaczenia wczesnej diagnozy. Trzeci przedstawia kierunki oddziaływań wspierających rozwój funkcji percepcyjno-motorycznych i językowych. W konkluzji podkreślono, że wczesna identyfikacja trudności oraz zintegrowane działania pedagogiczne i logopedyczne sprzyjają wyrównywaniu ewentualnych deficytów oraz zaakcentowano praktyczny wymiar działań profilaktycznych w edukacji przedszkolnej." (...)