Background: Urethral profilometry was developed as a scientific research method rather than for everyday clinical use. However, it was implemented as an integral part of urodynamic diagnostics for clinical use. Although it has been used for over 70 years, there are still no clear guidelines, either for the technique or for the indications and interpretation of the data obtained in this study. There are many inaccuracies and doubts about the method. Material and methods: The authors of this manuscript present a review of the options for urethral function diagnostics and the position of urethral profilometry among them. The literature and clinical aspects of their investigation are discussed in this article. The sources of the limitations of urethral profilometry and their importance in clinical practice and the interpretation of results are analyzed. Results: Urethral profilometry has many limitations. Their sources are urethral anatomy and structure, equipment, patient collaboration and the examination technique. The repeatability is debatable, but doubts surrounding it seem to be exaggerated. Despite all this many valuable data can be obtained by performing an examination. Conclusions: Despite the limitations, urethral profilometry is a useful clinical tool for functional diagnostics of the urethra. Developing precise guidelines regarding indications, technique and interpretation of the assessment would be essential in order to take advantage of this diagnostic method.